Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat4 (2A2) Mouse mAb #5097

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 81 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Stat4 (2A2) Mouse mAb detects endogenous levels of total Stat4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues 633-652 of human Stat4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected or transfected with human Stat4, using Stat4 (2A2) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from NK-92, TALL-104 and SR cells using Stat4 (2A2) Mouse mAb.

Background

The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9).

  1. Darnell, J.E. et al. (1994) Science 264, 1415-1421.
  2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
  3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810.
  4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342-4349.
  5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755-1762.
  6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311.
  7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174.
  8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.
  9. Visconti, R. et al. (2000) Blood 96, 1844-52.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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