Product Pathways - Cytoskeletal Signaling
RCC2 (D14F3) Rabbit mAb #5104
PhosphoSitePlus® protein, site, and accession data: TD-60
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 60 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
* Product-specific protocol.
Specificity / Sensitivity
RCC2 (D14F3) Rabbit mAb detects endogenous levels of total RCC2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RCC2 protein.
Western Blotting
Western blot analysis of extracts from various cell types using RCC2 (D14F3) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using RCC2 (D14F3) Rabbit mAb (green) and α-Tubulin (DM1A) Mouse mAb #3873 (red).
Background
RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC (reviewed in 1,2). In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B (3). RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres (4).
- Vader, G. et al. (2006) J Cell Biol 173, 833-7.
- Ruchaud, S. et al. (2007) Nat Rev Mol Cell Biol 8, 798-812.
- Mollinari, C. et al. (2003) Dev Cell 5, 295-307.
- Rosasco-Nitcher, S.E. et al. (2008) Science 319, 469-72.
Application References
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Companion Products
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
