Cell Signaling Technology

Product Pathways - Apoptosis

TAX1BP1 (D1D5) Rabbit mAb #5105

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 92 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TAX1BP1 (D1D5) Rabbit mAb recognizes endogenous levels of total TAX1BP1 protein. Based upon sequence alignment, this antibody is predicted to cross-react with TXBP151-L and TXBP151-S isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TAX1BP1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TAX1BP1 (D1D5) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding the full-length isoform 1 of human TAX1BP1 (hTAX1BP1-Myc/DDK, +), using TAX1BP1 (D1D5) Rabbit mAb.

Background

Tax1-binding protein (TAX1BP) 1 is an essential regulator of innate immunity and was originally identified in a yeast two-hybrid screen as a human T-lymphotropic virus Type 1 (HTLV-1) Tax1-binding protein and named TXBP151 (1-3). Independently, TAX1BP1 was discovered in yeast two-hybrid screens that sought to identify novel binding partners of A20 (4) and TRAF6, where it was named T6BP (5). Two human TAX1BP1 transcripts encoding modular proteins of 747 and 789 amino acids have been identified (4). The N-terminal region of TAX1BP1 possesses a SKIP carboxyl homology (SKITCH) domain and a 14-3-3 binding motif. The central region of TAX1BP1 harbors coiled-coil structures and helix-loop-helix regions that are thought to promote the formation of TAX1BP1 homodimers (5). The TAX1BP1 C-terminal region posesses zinc finger domains that function as novel ubiquitin-binding domains and allow for complex formation with K63-ubiquitinated RIP1 and TRAF6 (6) as well as the E3 ubiquitin ligase ITCH (7). One of the major physiologic roles of TAX1BP1 is to serve as an essential component of a negative feedback loop aimed at restraining canonical NF-κB-mediated proinflammatory signaling cascades initiated by TNF and IL-1. It is likely that TAX1BP1 functions as a ubiquitin-binding adaptor protein that inducibly recruits A20 to a complex consisting, in part, of K63-ubiquitinated TRAF6, RIP1, and their cognate E2 conjugating enzyme, thus allowing for A20-mediated ubiquitin-editing and termination of NF-κB signaling (6,8,9). A recent report identified IKKα as a novel regulator of TAX1BP1 function and demonstrated that IKKα-dependent phosphorylation of TAX1BP1 at Ser593 and Ser624 in response to TNF and IL-1 is critical for its ability to orchestrate formation of the A20 ubiquitin-editing complex involved in termination of NF-κB signaling (10).

  1. Chin, K.T. et al. (2007) Cancer Res 67, 1072-81.
  2. Wu, K. et al. (2004) J Biol Chem 279, 495-508.
  3. Gachon, F. et al. (1998) J Virol 72, 8332-7.
  4. De Valck, D. et al. (1999) Oncogene 18, 4182-90.
  5. Ling, L. and Goeddel, D.V. (2000) Proc Natl Acad Sci USA 97, 9567-72.
  6. Iha, H. et al. (2008) EMBO J 27, 629-41.
  7. Shembade, N. et al. (2009) EMBO J 28, 513-22.
  8. Shembade, N. et al. (2010) Science 327, 1135-9.
  9. Shembade, N. et al. (2007) EMBO J 26, 3910-22.
  10. Shembade, N. et al. (2011) Nat Immunol 12, 834-43.

Application References

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