Cell Signaling Technology

Product Pathways - MAPK Signaling

FAM129B Antibody #5122

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC F H Mk Endogenous 95 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FAM129B Antibody recognizes endogenous levels of total FAM129B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu705 of human Fam129B protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using FAM129B Antibody (upper) or GAPDH (14C10) Rabbit mAb Antibody #2118 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using FAM129B Antibody (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-431 (left) and HeLa cells (right) using FAM129B Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma (1,2). FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma (3,4). FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells (5). Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines (5). Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix (5).

  1. Sun, G.D. et al. (2007) Biochem Biophys Res Commun 360, 181-7.
  2. Matsumoto, F. et al. (2006) Hum Pathol 37, 1592-600.
  3. Boyd, R.S. et al. (2003) Leukemia 17, 1605-12.
  4. Han, M. et al. (2008) Clin Cancer Res 14, 455-60.
  5. Old, W.M. et al. (2009) Mol Cell 34, 115-31.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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