Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

TCTP (D10F2) Rabbit mAb #5128

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 23 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TCTP (D10F2) Rabbit mAb recognizes endogenous levels of total TCTP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCTP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TCTP (D10F2) Rabbit mAb.

Background

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12).

  1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
  2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
  3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
  4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
  5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
  6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
  7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
  8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
  9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
  10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
  11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
  12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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