Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RCC1 (D15H6) Rabbit mAb #5134

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Endogenous 45 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

RCC1 (D15H6) Rabbit mAb detects endogenous levels of total RCC1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RCC1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using RCC1 (D15H6) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using RCC1 (D15H6) Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red).

Background

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. Regulator of chromatin condensation 1 (RCC1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclin B/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and RCC1 RanGEF activity (6).

  1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
  2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
  3. Moore, W. et al. (2002) Curr Biol 12, 1442-7.
  4. Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97.
  5. Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27.
  6. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.
  7. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603.
  8. Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45.
  9. Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23.
  10. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
  11. Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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