Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Myosin II Isoform Antibody Sampler Kit #5144

Kit Includes Quantity Applications Reactivity MW (kDa) Isotype
Myosin IIa Antibody #3403 40 µl W IF-IC H M R 230 Rabbit
Myosin IIb Antibody #3404 40 µl W IF-IC H M Mk (R) 230 Rabbit
Myosin IIc Antibody #3405 40 µl W H M Mk 230 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl Goat

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Specificity / Sensitivity

Each antibody in the Myosin II Isoform Antibody Sampler Kit detects endogenous total levels of the specified myosin II isoform.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Myosin IIa Antibody #3403.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Myosin IIb Antibody #3404.

Western Blotting

Western Blotting

Western blot analysis of extracts from from COS cells, untransfected (mock) or transfected with nonmuscle EGFP-myosin IIa, IIb or IIc, using Myosin IIc Antibody #3405.


Description

The Myosin II Isoform Antibody Sampler Kit is an economical way to examine the total protein levels of myosin II isoforms a, b, and c. The kit includes enough primary and secondary antibodies to perform four Western blot experiments.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the carboxy terminus of mouse myosin IIa and human myosin IIb and IIc.

Background

Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).

  1. Conti, M.A. and Adelstein, R.S. (2008) J Cell Sci 121, 11-18.
  2. Sandquist, J.C. et al. (2006) J Biol Chem 281, 35873-83.
  3. Even-Ram, S. et al. (2007) Nat Cell Biol 9, 299-309.
  4. Vicente-Manzanares, M. et al. (2007) J Cell Biol 176, 573-80.
  5. Wylie, S.R. and Chantler, P.D. (2008) Mol Biol Cell 19, 3956-68.
  6. Sandquist, J.C. and Means, A.R. (2008) Mol Biol Cell 19, 5156-67.
  7. Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18, 3144-55.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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