Product Pathways - Cytoskeletal Signaling
Vav Isoform Antibody Sampler Kit #5145
| Kit Includes | Quantity | Applications | Reactivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| Vav1 (D45G3) Rabbit mAb #4657 | 40 µl | W IP | H (Mk) | 95 | Rabbit IgG |
| Vav2 (C64H2) Rabbit mAb #2848 | 40 µl | W IP | H Mk | 100 | Rabbit IgG |
| Vav3 Antibody #2398 | 40 µl | W | H | 98 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Each antibody in the Vav Isoform Antibody Sampler Kit detects endogenous levels of the specified Vav isoform.
Western Blotting
Western blot analysis of extracts from Jurkat, MCF-7 and HUVEC cells using Vav3 Antibody #2398.
Western Blotting
Western blot analysis of extracts from various cell types using Vav2 (C64H2) Rabbit mAb #2848.
Western Blotting
Western blot analysis of extracts from Jurkat, Raji and K-562 cells using Vav1 (D45G3) Rabbit mAb #4657.
Description
The Vav Isoform Antibody Sampler Kit is an economical way to examine the total protein levels of Vav isoforms 1, 2, and 3. The kit includes enough primary and secondary antibodies to perform four Western blot experiments.
Source / Purification
Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human Vav3. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy terminus of Vav1 and near the carboxy terminus of human Vav2.
Background
Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).
- Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
- Crespo, P. et al. (1997) Nature 385, 169-172.
- Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.
Application References
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Protocols
- 2398:
- Western Blotting
- 2848:
- Immunoprecipitation, Western Blotting
- 4657:
- Immunoprecipitation, Western Blotting
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
