Cell Signaling Technology

Product Pathways - MAPK Signaling

HGK (D19H10) Rabbit mAb #5146

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Mk Endogenous 150 kDa Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HGK (D19H10) Rabbit mAb detects endogenous levels of total HGK protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala553 of human HGK protein.

Western Blotting

Western Blotting

Western blot analsysis of extracts from various cell lines using HGK (D19H10) Rabbit mAb.

Background

HGK (MAP4K4 or mitogen-activated protein kinase kinase kinase kinase 4) is a serine/threonine kinase that belongs to the mammalian STE20/MAP4K kinase family involved in response to environmental stress and cytokines such as TNF-α (1-3). HGK specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway and increases AP-1-mediated transcriptional activity in vivo (1). HGK is broadly expressed in many types of human cancer and cancer cell lines and plays an important role in cell transformation, invasiveness, adhesion and migration (4,5).

  1. Yao, Z. et al. (1999) J Biol Chem 274, 2118-25.
  2. Tesz, G.J. et al. (2007) J Biol Chem 282, 19302-12.
  3. Bouzakri, K. and Zierath, J.R. (2007) J Biol Chem 282, 7783-9.
  4. Wright, J.H. et al. (2003) Mol Cell Biol 23, 2068-82.
  5. Collins, C.S. et al. (2006) Proc Natl Acad Sci USA 103, 3775-80.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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