Product Pathways - MAPK Signaling
SRF (D71A9) XP® Rabbit mAb #5147
|5147S||100 µl (10 western blots)||---||In Stock||---|
|5147P||40 µl (4 western blots)||---||In Stock||---|
|5147||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey, Pig||Endogenous||67||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP
Specificity / Sensitivity
SRF (D71A9) XP® Rabbit mAb recognizes endogenous levels of total SRF protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser375 of human SRF protein.
Western blot analysis of extracts from various cell lines using SRF (D71A9) XP® Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells starved overnight and treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (100 ng/ml) for 3 hours, and either 5 μl of SRF1 (D71A9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6).
- Treisman, R. (1995) EMBO J. 14, 4905-4913.
- Janknecht, R. et al. (1995) Carcinogenesis 16, 443-450.
- Marais, R. M. et al. (1992) EMBO J. 11, 97-105.
- Rivera, V. M. et al. (1993) Mol. Cell. Biol. 13, 6260-6273.
- Miranti, C. K. et al. (1995) Mol. Cell. Biol. 15, 3672-3684.
- Heidenreich, O. et al. (1999) JBC 274, 14434-14443.
- Xifró, X. et al. (2011) Mol Neurodegener 6, 74. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 4261 Phospho-SRF (Ser103) Antibody
- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 4663 SimpleChIP® Human c-Fos Promoter Primers
- 4486 SimpleChIP® Human α Satellite Repeat Primers
- 2729 Normal Rabbit IgG
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4174 TPA (12-O-Tetradecanoylphorbol-13-Acetate)
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.