Product Pathways - Nuclear Receptor Signaling
Androgen Receptor (D6F11) XP® Rabbit mAb #5153
PhosphoSitePlus® protein, site, and accession data: AR
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 110 | Rabbit IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Androgen Receptor (D6F11) XP® Rabbit mAb detects endogenous levels of total androgen receptor protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein.
Western Blotting
Western blot analysis of extracts from LNCaP (AR+), MCF7 (AR+), PC-3 (AR-), and DU 145 (AR-) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (upper) and β-Actin Antibody #4967 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP (AR+, left) and DU145 (AR-, right) using Androgen Receptor (D6F11) XP® Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Androgen Receptor (D6F11) XP® Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of DU-145 cells (red) and LNCaP cells (blue) using Androgen Receptor (D6F11) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of LNCaP (positive, left) and DU145 (negative, right) cells using Androgen Receptor (D6F11) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
- Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
- Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
- Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
