Cell Signaling Technology

Product Pathways - Development

Frizzled6 (D16E5) Rabbit mAb #5158

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 75-85 kDa Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Frizzled6 (D16E5) Rabbit mAb detects endogenous levels of total Frizzled6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Frizzled6 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Frizzled6 siRNA I #6596, 100 nM SignalSilence® Frizzled6 siRNA II #6597, or mock transfected, using Frizzled6 (D16E5) Rabbit mAb (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Frizzled6 (D16E5) Rabbit mAb confirms silencing of Frizzled6 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.

Western blot analysis of extracts from 786-0, HeLa, and HCT 116 cells using Frizzled6 (D16E5) Rabbit mAb.

Background

Frizzled (Fzd) belongs to the seven transmembrane-spanning G-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca2+ pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11).

  1. Schulte, G. and Bryja, V. (2007) Trends Pharmacol Sci 28, 518-25.
  2. Hsieh, J.C. et al. (1999) Proc Natl Acad Sci USA 96, 3546-51.
  3. Wong, H.C. et al. (2003) Mol Cell 12, 1251-60.
  4. Zeng, X. et al. (2005) Nature 438, 873-7.
  5. Davidson, G. et al. (2005) Nature 438, 867-72.
  6. Fanto, M. and McNeill, H. (2004) J Cell Sci 117, 527-33.
  7. Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46.
  8. Cadigan, K.M. and Liu, Y.I. (2006) J Cell Sci 119, 395-402.
  9. Xu, Q. et al. (2004) Cell 116, 883-95.
  10. Nam, J.S. et al. (2006) J Biol Chem 281, 13247-57.
  11. Hendrickx, M. and Leyns, L. (2008) Dev Growth Differ 50, 229-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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