Cell Signaling Technology

Product Pathways - DNA Damage

MYH Antibody #5159

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 55 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MYH Antibody detects endogenous levels of total MYH protein. Based on the amino acid sequence, the antibody is expected to react with all isoforms of human MYH protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy terminal residues of human MYH protein. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using MYH Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected (-) or MYH-transfected (+), using MYH Antibody.

Background

Base excision repair (BER) proteins catalyze the removal of incorrect or damaged bases, including oxidized bases, from DNA. N-glycosylases specific to a given lesion remove the incorrect base as the first step in BER. MYH is the mammalian ortholog of E. coli MutY, a DNA glycosylase that catalyzes the removal of 8-oxoG:A mismatches (1). Several MYH isoforms have been detected in human cells localizing to either the nucleus or the mitochondria (2). MYH interacts with DNA repair proteins and localizes to DNA damage foci after oxidative damage (3). Research studies have shown that mutations in the corresponding MYH gene are associated with human gastric (4) and colorectal (5-7) cancers.

  1. Slupska, M.M. et al. (1996) J Bacteriol 178, 3885-92.
  2. Ohtsubo, T. et al. (2000) Nucleic Acids Res 28, 1355-64.
  3. Shi, G. et al. (2006) Biochem J 400, 53-62.
  4. Kobayashi, K. et al. (2008) Anticancer Res 28, 215-21.
  5. Bai, H. et al. (2007) Cancer Lett 250, 74-81.
  6. Pope, M.A. et al. (2005) DNA Repair (Amst) 4, 315-25.
  7. Wooden, S.H. et al. (2004) Cancer Lett 205, 89-95.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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