Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

EAPP (1E4) Mouse mAb #5166

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Endogenous 45 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EAPP (1E4) Mouse mAb detects endogenous levels of total EAPP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant amino-terminal fragment of human EAPP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using EAPP (1E4) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells using EAPP (1E4) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Regulation of the E2F family of transcription factors, primarily through the retinoblastoma protein (pRb), is vital for control of cell proliferation and cell death (reviewed in 1). E2F-associated phosphoprotein (EAPP) was identified as an E2F-family binding protein that modulates E2F-regulated transcription and may be required for S phase entry. EAPP is expressed at varied levels in all tissues and cell types examined and its expression is reduced in nocodazole-arrested cells (2). Mass spectrometry studies have identified multiple EAPP phosphorylation sites including Ser109 and Ser111, but biological consequences of EAPP phosphorylation have yet to be elucidated (3-5).

  1. Polager, S. and Ginsberg, D. (2008) Trends Cell Biol 18, 528-35.
  2. Novy, M. et al. (2005) Mol Biol Cell 16, 2181-90.
  3. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
  4. Mayya, V. et al. (2009) Sci Signal 2, ra46.
  5. Olsen, J.V. et al. (2006) Cell 127, 635-48.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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