Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Translational Control

eIF4G2/p97 (D88B6) XP® Rabbit mAb #5169

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H M R Mk Endogenous 97 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4G2/p97 (D88B6) XP® Rabbit mAb detects endogenous levels of total eIF4G2/p97 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu450 of human eIF4G2/p97 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using eIF4G2/p97 (D88B6) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using eIF4G2/p97 (D88B6) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using eIF4G2/p97 (D88B6) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).

  1. Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
  2. Morley, S.J. et al. (1997) RNA 3, 1085-1104.
  3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
  4. De Gregorio, E. et al. (1998) RNA 4, 828-836.
  5. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
  6. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
  7. Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405.
  8. Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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