Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

Cofilin (D3F9) XP® Rabbit mAb #5175

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk Dg Endogenous 19 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Cofilin (D3F9) XP® Rabbit mAb detects endogenous levels of total cofilin1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human cofilin1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Cofilin (D3F9) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Cofilin (D3F9) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

  1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
  2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
  3. Arber, S. et al. (1998) Nature 393, 805-9.
  4. Yang, N. et al. (1998) Nature 393, 809-12.
  5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
  6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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