Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-CARD11 (Ser652) Antibody #5189

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-CARD11 (Ser652) Antibody detects endogenous levels of CARD11 protein only when phosphorylated at Ser652.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to a region surrounding Ser652 of human CARD11 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with TPA #9905 (40 nM) and calcium ionophore A23187 (2 μM) for 15 minutes, and λ-phosphatase plus calf intestinal phosphatase (CIP) as indicated, using Phospho-CARD11 (Ser652) Antibody (upper) and CARD11 (1D12) Rabbit mAb #4435 (lower).

Background

CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7).

Phosphorylation at multiple sites within the central region of CARD11 regulates NF-κB activation (8-10).

  1. Bertin, J. et al. (2001) J. Biol. Chem. 276, 11877–11882.
  2. Gaide, O. et al. (2001) FEBS Lett. 496, 121-127.
  3. Stilo, R. et al. (2004) J. Biol. Chem. 279, 34323-34331.
  4. Wang, D. et al. (2002) Nat. Immunol. 3, 830-835.
  5. Jun, J.E. et al. (2003) Immunity 18, 751-762.
  6. Hara, H. et al. (2003) Immunity 18, 763-775.
  7. Gaide, O. et al. (2002) Nat. Immunol. 3, 836-843.
  8. Sommer, K. et al. (2005) Immunity 23, 561-74.
  9. Shinohara, H. et al. (2007) J Exp Med 204, 3285-93.
  10. Moreno-García, M.E. et al. (2009) J Immunol 183, 7362-70.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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