Cell Signaling Technology

Product Pathways - Metabolism

Lipin 1 Antibody #5195

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H M Endogenous 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Lipin 1 Antibody detects endogenous levels of total Lipin 1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln883 of human Lipin 1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human white pre-adipocytes and human white adipocytes using Lipin 1 Antibody (upper) and β-Actin Antibody #4967 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of 3T3-L1 adipocytes using Lipin 1 Antibody (red). Lipid droplets were labeled with BODIPY® 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Lipin 1 was identified as a nuclear protein required for adipose tissue development (1). The expression of Lipin 1 is induced during adipocyte differentiation (1). The abnormal development of adipose tissues caused by mutations in the lipin 1 gene results in lipodystrophy, a condition associated with low body fat, fatty liver, hypertriglyceridemia, and insulin resistance (1). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (2-4). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in liver, and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (5). Lipin1 is regulated by mTOR, illustrating a connection between adipocyte development and nutrient-sensing pathways (6). It also mediates hepatic insulin signaling by TORC2/CRTC2 (7).

  1. Péterfy, M. et al. (2001) Nat Genet 27, 121-4.
  2. Finck, B.N. et al. (2006) Cell Metab 4, 199-210.
  3. Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83.
  4. Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64.
  5. Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6.
  6. Huffman, T.A. et al. (2002) Proc Natl Acad Sci U S A 99, 1047-52.
  7. Ryu, D. et al. (2009) Cell Metab 9, 240-51.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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