Cell Signaling Technology

Product Pathways - NF-kB Signaling

Phospho-IκBα (Ser32/36) (12C2) Mouse mAb #5210

Applications Reactivity MW (kDa) Isotype
E-P All 40 Mouse IgG2a

Applications Key:  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Specificity / Sensitivity

Phospho-IκBα (Ser32/36) (12C2) Monoclonal Antibody can be used in high throughput kinase assays and drug discovery applications. It detects peptides derived from IκBα phosphorylated at Ser32/36.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα. Antibody is purified by protein G chromatography.

ELISA-Peptide

ELISA-Peptide

Validation of Phospho-IkBα (Ser32/36)(12C2) Monoclonal Antibody in a peptide DELFIA® assay, using biotinylated phospho-, nonphospho-peptide controls, and DELFIA® secondary antibodies (available from PerkinElmer Life and Analytical Sciences).

Description

This antibody is formulated in PBS (no BSA/no glycerol) and quality controlled for use in ELISA and other drug discovery applications. This is a sample antibody and intended for use by drug discovery scientists.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

  1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
  2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
  3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
  4. Brown, K. et al. (1995) Science 267, 1485-8.
  5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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