Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

TBX21 (V365) Antibody #5214

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 65 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TBX21 (V365) Antibody detects endogenous levels of total TBX21 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val365 of human TBX21 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western analysis of extracts from TALL-104 cells using TBX21 (V365) Antibody.

Background

The T-box gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The T-box genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in T-box genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of T-box family (7-9).

TBX21, also as known as T-bet, plays a critical role in development and maintenance of type 1 helper T (Th1) and TBX21 deficient mice display impaired Th1 differentiation (10,11).

  1. Wilkinson, D.G. et al. (1990) Nature 343, 657-9.
  2. Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20, 9-19.
  3. Showell, C. et al. (2004) Dev Dyn 229, 201-18.
  4. Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70.
  5. Hoogaars, W.M. et al. (2007) Cell Mol Life Sci 64, 646-60.
  6. Baldini, A. (2004) Curr Opin Cardiol 19, 201-4.
  7. Abrahams, A. et al. (2010) IUBMB Life 62, 92-102.
  8. Rowley, M. et al. (2004) J Mammary Gland Biol Neoplasia 9, 109-18.
  9. Yang, X.R. et al. (2009) Nat Genet 41, 1176-8.
  10. Ho, I.C. and Glimcher, L.H. (2002) Cell 109 Suppl, S109-20.
  11. Peng, S.L. (2006) Cell Mol Immunol 3, 87-95.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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