Product Pathways - Development
Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232
PhosphoSitePlus® protein, site, and accession data: NANOG
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| ChIP | H | Endogenous | 42 | Rabbit IgG |
Applications Key:
ChIP=Chromatin IP
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5232:
- ChIP Agarose, ChIP Magnetic
Specificity / Sensitivity
Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) detects endogenous levels of total nanog protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human nanog protein.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and 10 μl of Nanog, Oct-4 and Sox2 antibodies, or 2 μl of Normal Rabbit IgG, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background
Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).
- Kim, J. et al. (2008) Cell 132, 1049-61.
- Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
- Yu, J. et al. (2007) Science 318, 1917-20.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4903 Nanog (D73G4) XP® Rabbit mAb
- 5024 Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated)
- 5677 Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated)
- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9007 ChIP-Grade Protein G Agarose Beads
- 9006 ChIP-Grade Protein G Magnetic Beads
- 7017 6-Tube Magnetic Separation Rack
For Research Use Only. Not For Use In Diagnostic Procedures.
