Product Pathways - Growth Factors/Cytokines
Human Interleukin-1α (hIL-1α) #5236
Recombinant human IL-1α (hIL-1α) Ser113-Ala271 (Accession #NP_000566) was produced in E. coli at Cell Signaling Technology.
Recombinant hIL-1α does not have a Met on the amino terminus and has a calculated MW of 18,063. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal SSPFS of recombinant hIL-1α was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1α. All lots are greater than 98% pure.
The bioactivity of recombinant hIL-1α was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-15 pg/ml.
The purity of recombinant hIL-1α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-1α and staining overnight with Coomassie Blue.
The production of human IL-8 by primary human fibroblasts cultured with increasing concentrations of hIL-1α was assessed. Media from cells incubated with hIL-1α for 24 hours was collected and assayed for human IL-8 by ELISA and the OD450-OD650 was determined.
Western blot analysis of extracts from MCF7 cells, untreated or treated with hIL-1α for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (E498) Antibody #3987 (lower).
Less than 0.01 ng endotoxin/1 μg hIL-1α.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT and 20 μg BSA per 1 μg hIL-1α. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 4mM DTT.
IL-1α is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes, and epithelial cells (1). IL-1α is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2). Often, precursor and mature forms of IL-1α are primarily retained intracellularly rather than constitutively secreted. (1,2). Signaling by IL-1α involves IL-1α binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1α also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1α activity is inhibited through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal. IL-1α has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).
- Dinarello, C.A. (1996) Blood 87, 2095-147.
- Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.
- Di Paolo, N.C. et al. (2009) Immunity 31, 110-21.
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