Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-TCTP (Ser46) Antibody #5251

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P IF-IC F H M R Mk Endogenous 23 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-TCTP (Ser46) Antibody recognizes endogenous levels TCTP protein only when phosphorylated at Ser46. In some cell types the antibody may recognize an 80-100 kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser46 of human TCTP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U-2 OS cells, untreated or synchronized in mitosis by thymidine block followed by release into nocodazole, using Phospho-TCTP (Ser46) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda-phosphatase-treated (right), using Phospho-TCTP (Ser46) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or nocodazole-treated (right), using Phospho-TCTP (Ser46) Antibody.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-TCTP (Ser46) Antibody compared to propidium iodide (DNA content).

IF-IC

IF-IC

Confocal immunofluorescent analysis of U-2 OS cells using Phospho-TCTP1 (Ser46) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Translationally controlled tumor protein (TCTP/p23/HRF) is a ubiquitously expressed and highly conserved protein involved in various cellular processes, such as its role as a histamine releasing factor in chronic allergic disease (1). TCTP binds tubulin in a cell cycle dependent manner and is associated with the mitotic spindle (2). In addition, TCTP interacts with the actin cytoskeleton to regulate cell shape (3). In mitosis, TCTP is phosphorylated by PLK at Ser46, decreasing microtubule stability (4,5). TCTP interacts with the small GTPase Rheb, possibly acting as a GEF, thereby activating the TORC1 pathway and controlling cell growth and proliferation (6,7). TCTP has also been shown to be involved in apoptosis and cell stress (8-11). In cultured cells, reduction in TCTP expression can cause loss of the malignant phenotype (12).

  1. MacDonald, S.M. et al. (1995) Science 269, 688-90.
  2. Gachet, Y. et al. (1999) J Cell Sci 112 ( Pt 8), 1257-71.
  3. Bazile, F. et al. (2009) Carcinogenesis 30, 555-65.
  4. Yarm, F.R. (2002) Mol Cell Biol 22, 6209-21.
  5. Dephoure, N. et al. (2008) Proc Natl Acad Sci USA 105, 10762-7.
  6. Dong, X. et al. (2009) J Biol Chem 284, 23754-64.
  7. Hsu, Y.C. et al. (2007) Nature 445, 785-8.
  8. Susini, L. et al. (2008) Cell Death Differ 15, 1211-20.
  9. Bommer, U.A. et al. (2009) Oncogene [Epub ahead of print].
  10. Gnanasekar, M. et al. (2009) Biochem Biophys Res Commun 386, 333-7.
  11. Gnanasekar, M. et al. (2009) Int J Oncol 34, 1241-6.
  12. Tuynder, M. et al. (2002) Proc Natl Acad Sci USA 99, 14976-81.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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