Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

SH2D1A (D10G8) Rabbit mAb #5272

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M (R) Endogenous 14 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SH2D1A (D10G8) Rabbit mAb detects endogenous levels of total SH2D1A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala82 of human SH2D1A protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and MOLT-4 cells using SH2D1A (D10G8) Rabbit mAb.

Background

SH2D1A and SH2D1B are small, adaptor proteins with a single SH2-domain that play important signal transduction roles mediated by the signaling lymphocytic activation molecule (SLAM) family receptors (1). SH2D1A (also called SAP or SLAM-associated protein) is frequently mutated in patients with X-linked lymphoproliferative disease (Duncan’s disease), which is characterized by extreme susceptibility to Epstein-Barr virus; approximately 50 different SH2D1A mutations have been reported to date (2-4). The single SH2D1B gene in humans (also called EAT-2 or Ewing's sarcoma's/FLI1-activated transcript 2) is present as a pair of duplicated EAT-2A and EAT-2B genes with identical genomic organization in mouse and rat (5,6).

  1. Latour, S. and Veillette, A. (2004) Semin. Immunol. 16, 409-419.
  2. Nichols, K.E. et al. (2005) Immunol. Rev. 203, 180-199.
  3. Engel, P. et al. (2003) Nat. Rev. Immunol. 3, 813-821.
  4. Sumegi, J. et al. (2002) Leuk. Lymphoma 43, 1189-1201.
  5. Roncagalli, R. et al. (2005) Nat. Immunol. 6, 1002-1010.
  6. Calpe, S. et al. (2006) Immunogenetics 58, 15-25.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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