Product Pathways - Ca / cAMP / Lipid Signaling
Gα(i) Antibody #5290
|5290S||100 µl (10 western blots)||---||In Stock||---|
|5290||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||40||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Gα(i) Antibody detects endogenous levels of total Gα(i) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg100 of human Gα(i). Antibodies are purified by protein A and peptide affinity chromatography.
Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).
Gα(i) causes inhibition of adenylate cyclase, leading to a decrease in cellular levels of cAMP. Pertussis toxin catalyzes ADP-ribosylation of Gα(i), which inactivates the Gα(i) protein and attenuates inhibition of adenylate cyclase (4).
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