Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

FoxP3 (D25D4) Rabbit mAb #5298

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 45 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxP3 (D25D4) Rabbit mAb detects endogenous levels of total FoxP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp268 of human FoxP3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected or transfected with human FoxP3, and human thymus, using FoxP3 (D25D4) Rabbit mAb.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immunodeficiency polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). In addition, FoxP3 functions as a tumor suppressor in several types of cancer (9-11).

  1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
  2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
  3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
  4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
  5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
  6. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
  7. Bennett, C.L. et al. (2001) Nat Genet 27, 20-1.
  8. Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23.
  9. Zuo, T. et al. (2007) Cell 129, 1275-86.
  10. Zuo, T. et al. (2007) J Clin Invest 117, 3765-73.
  11. Wang, L. et al. (2009) Cancer Cell 16, 336-46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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