Cell Signaling Technology

Product Pathways - PathScan Multiplex WB Cocktails

PathScan® Bcr/Abl Activity Assay: Phospho-c-Abl, Phospho-Stat5 and Phospho-CrkL Multiplex Western Detection Cocktail II #5300

Applications Reactivity Source
W H Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

This Cocktail Contains The Following Antibodies:

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells untreated or STI-571 treated, using PathScan Bcr/Abl Activity Assay cocktail to detect inhibition of phospho-Bcr-Abl, phospho-Stat5 and phospho-CrkL.

Description

The PathScan® Multiplex Western Detection Cocktail offers a unique method to assay the inhibition of multiple proteins on one membrane without stripping and reprobing. This method saves the user valuable time while increasing accuracy and minimizing reagent waste. The Bcr/Abl Activity Assay allows the user to simultaneously detect the inhibition of phosphorylation of c-Abl, Stat5 and CrkL proteins in response to STI-571. The cocktail also includes elF4E antibody to control protein loading.

Specificity / Sensitivity

Each phospho-antibody in this cocktail recognizes endogenous levels of only the phosphorylated form of its specific target. The eIF4E Antibody detects endogenous levels of its target protein independent of phosphorylation and is provided to control for protein loading.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with synthetic peptides. Antibodies are purified by protein A and peptide affinity chromatography.

Background

STI-571 (also known as Imatinib mesylate and Gleevec™) is a tyrosine kinase (TK) inhibitor that is a relatively specific ATP-binding site antagonist of Bcr-Abl, PDGF receptor and c-Kit TKs (1-3). Results are encouraging in CML clinical trials, and STI-571 has become a paradigm for targeted cancer therapeutics (4-6). Signal transduction through phospho-tyrosine pathways has been studied extensively, and tyrosine phosphorylation has been linked to multiple cell growth and differentiation pathways (7-9). Because the observed leukemic state of CML is dependent on the intact Bcr-Abl tyrosine kinase activity, extensive work has been done to identify substrates of Bcr-Abl and thus possible mechanisms leading to a myeloid expansion. Many groups have characterized prominent tyrosine-phosphorylated protein substrates in both CML blasts and Bcr-Abl-expressing cell lines, including SHIP, c-cbl, Dok, SHC and CrkL (10-15). In addition, key signal transduction pathways involving PI3 kinase, Ras, Myc and Stat5 are also activated in a Bcr-Abl kinase-dependent manner (16).

  1. Buchdunger, E. et al. (1996) Cancer Res 56, 100-4.
  2. Heinrich, M.C. et al. (2000) Blood 96, 925-32.
  3. Druker, B.J. et al. (1996) Nat Med 2, 561-6.
  4. Mauro, M.J. and Druker, B.J. (2001) Curr Oncol Rep 3, 223-7.
  5. Druker, B.J. et al. (2001) N Engl J Med 344, 1031-7.
  6. Druker, B.J. et al. (2001) N Engl J Med 344, 1038-42.
  7. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  8. Ullrich, A. and Schlessinger, J. (1990) Cell 61, 203-12.
  9. Cantley, L.C. et al. (1991) Cell 64, 281-302.
  10. ten Hoeve, J. et al. (1994) Blood 84, 1731-6.
  11. Matsuguchi, T. et al. (1994) J Biol Chem 269, 5016-21.
  12. Carpino, N. et al. (1997) Cell 88, 197-204.
  13. Sattler, M. et al. (1997) Oncogene 15, 2379-84.
  14. Di Cristofano, A. et al. (1998) J Biol Chem 273, 4827-30.
  15. Wisniewski, D. et al. (1999) Blood 93, 2707-20.
  16. Kabarowski, J.H. and Witte, O.N. (2000) Stem Cells 18, 399-408.

Application References

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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