Product Pathways - MAPK Signaling
PathScan® PDGFR Activity Assay: Phospho-PDGFR, Phospho-SHP2, Phospho-Akt, and Phospho-p44/42 MAPK (Erk1/2) Multiplex Western Detection Cocktail II #5304
|5304S||250 µl (5 western blots)||---||In Stock||---|
|5304||carrier free and custom formulation / quantity||email request|
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|W||1:200||Human, Mouse, Rat||Endogenous||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Antibodies In This Cocktail
- Phospho-PDGF Receptor β (Tyr751) Antibody #3161
- Phospho-SHP-2 (Tyr542) Antibody #3751
- Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058
- Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370
- eIF4E (C46H6) Rabbit mAb #2067
The PathScan® Multiplex Western Detection Cocktail offers a unique method to assay the activation of multiple proteins on one membrane without stripping and reprobing. This method saves the user valuable time while increasing accuracy and minimizing reagent waste. The PDGFR Tyrosine Kinase Activity Assay allows the user to simultaneously detect the phosphorylation of PDGF receptor, SHP2, Akt and p44/42 MAPK proteins in response to PDGF. The kit also includes an eIF4E antibody to control protein loading.
Specificity / Sensitivity
Each phospho-antibody in this cocktail recognizes endogenous levels of only the phosphorylated form of its specific target. The eIF4E antibody detects endogenous levels of its target independent of phosphorylation and is provided to control for protein loading.
Source / Purification
Antibodies are produced by immunizing animals with synthetic peptides, and are purified by combinations of Protein A and peptide affinity chromatography.
Platelet-derived growth factor (PDGF) is a dimeric molecule that exists as homodimers or heterodimers of related polypeptide chains (A and B). Two types of PDGF receptors have been identified. The PDGF alpha-receptor binds all three isoforms with high affinity, whereas the beta-receptor binds only PDGF-BB with high affinity, PDGF-AB with low affinity and does not appear to bind PDGF-AA (1). PDGF exerts its stimulatory effects on cells by binding to these two related protein tyrosine kinase receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2-domain-containing signal transduction molecules. Thereby, a number of different signaling pathways are initiated, leading to cell growth, actin reorganization, migration and differentiation (2-4). In clinical studies, PDGF expression has been shown in a number of different solid tumors, from glioblastomas to prostate carcinomas. In these various tumor types, the biologic role of PDGF signaling can vary from autocrine stimulation of cancer cell growth to more subtle paracrine interactions involving adjacent stroma and even angiogenesis. Targeting PDGF signaling becomes an effective way for tumor treatment (5).
- Westermark, B. et al. (1990) Ciba Found. Symp. 150, 6-22.
- Heldin, C.H. (1997) FEBS Lett. 410, 17-21.
- Bornfeldt, K. E. et al. (1995) Ann. N. Y. Acad. Sci. 766, 416-430.
- Renhowe, P.A. (2002) Curr. Opin. Drug Discov. Devel. 5, 214-224.
- George, D. (2001) Semin. Oncol. 28, 27-33.
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- 4549 Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb
- 3751 Phospho-SHP-2 (Tyr542) Antibody
- 4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
- 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
- 2067 eIF4E (C46H6) Rabbit mAb
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
PathScan® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.