Cell Signaling Technology

Product Pathways - Neuroscience

GAP43 Antibody #5307

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 38, 43 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GAP43 Antibody detects endogenous levels of total GAP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GAP43 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using GAP43 Antibody.

Background

GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's disease (6,7).

  1. Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
  2. Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
  3. Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
  4. Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
  5. Shen, Y. et al. (2008) Cerebellum 7, 451-66.
  6. Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
  7. de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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