Cell Signaling Technology

Product Pathways - Phosphatases

PTP1B Antibody #5311

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 50 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PTP1B Antibody detects endogenous levels of total PTP1B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PTP1B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PTP1B Antibody.

Background

Protein-tyrosine phosphatase 1B (PTP1B) is the original member of the protein tyrosine phosphatase (PTP) family of proteins (1). PTP1B is highly expressed in insulin-responsive tissues and negatively regulates insulin, as well as integrin, through dephosphorylation of phosphotyrosine residues (2-4). PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes (5).

  1. Liang, F. et al. (2005) J Biol Chem 280, 24857-63.
  2. Kenner, K.A. et al. (1996) J Biol Chem 271, 19810-6.
  3. Klaman, L.D. et al. (2000) Mol Cell Biol 20, 5479-89.
  4. Liu, F. et al. (1998) Curr Biol 8, 173-6.
  5. Elchebly, M. et al. (1999) Science 283, 1544-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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