Cell Signaling Technology

Product Pathways - Metabolism

GFAT1 (D12F4) Rabbit mAb #5322

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H R Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GFAT1 (D12F4) Rabbit mAb detects endogenous levels of total GFAT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Gly100 of human GFAT1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using GFAT1 (D12F4) Rabbit mAb.

Background

GFAT1, glutamine:fructose-6-phosphate aminotransferase 1, is the rate-limiting enzyme of the hexosamine biosynthesis pathway (1). This enzyme catalyzes the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate and glutamate (2). The hexosamine biosynthesis pathway generates the building blocks for protein and lipid glycosylation (2). Furthermore, studies suggest that increased activity of this pathway is a contributing factor to hyperglycemia-induced insulin resistance (1,2). GFAT1 is more active in non-insulin-dependent diabetes mellitus (NIDDM) patients (3). Transgenice mice overexpressing this enzyme in skeletal muscle and adipose tissue show an insulin resistance phenotype (4,5). GFAT2, an isoenzyme of GFAT1, was later identified (6, 7). Studies show that the regulation of GFAT2 is different from that of GFAT1, suggesting differential regulation of the hexosamine pathway in different tissues (7).

  1. Niimi, M. et al. (2001) J Hum Genet 46, 566-71.
  2. DeHaven, J.E. et al. (2001) Diabetes 50, 2419-24.
  3. Yki-Järvinen, H. et al. (1999) Life Sci 65, 215-23.
  4. Cooksey, R.C. et al. (1999) Endocrinology 140, 1151-7.
  5. Hebert, L.F. et al. (1996) J Clin Invest 98, 930-6.
  6. Oki, T. et al. (1999) Genomics 57, 227-34.
  7. Hu, Y. et al. (2004) J Biol Chem 279, 29988-93.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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