Cell Signaling Technology

Product Pathways - Development

FoxP2 (D55H9) Rabbit mAb #5337

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H (M) (R) Endogenous 85 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FoxP2 (D55H9) Rabbit mAb detects endogenous levels of total FoxP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu623 of human FoxP2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells using FoxP2 (D55H9) Rabbit mAb.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP2 regulates lung and nerve system development (6-10). Mutations, including point mutation, truncation, deletion, and chromosomal translocation, involving FoxP2 are associated with an inherited form of speech and language disorder (11,12).

  1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
  2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
  3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
  4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
  5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
  6. Shu, W. et al. (2007) Development 134, 1991-2000.
  7. Yang, Z. et al. (2010) Life Sci 87, 17-25.
  8. Konopka, G. et al. (2009) Nature 462, 213-7.
  9. Enard, W. et al. (2009) Cell 137, 961-71.
  10. Morikawa, Y. et al. (2009) Neuroscience 162, 1150-62.
  11. Scharff, C. and White, S.A. (2004) Ann N Y Acad Sci 1016, 325-47.
  12. Fisher, S.E. and Scharff, C. (2009) Trends Genet 25, 166-77.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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