Cell Signaling Technology

Product Pathways - Metabolism

ASCT2 (V501) Antibody #5345

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC F H M R Endogenous 49, 75 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ASCT2 (V501) Antibody detects endogenous levels of total ASCT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ASCT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated or treated with peptide N-glycosidase F (PNGase F), and untreated SW620 cells, using ASCT2 (V501) Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT-29 cells using ASCT2 (V501) Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells using ASCT2 (V501) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6).

  1. Utsunomiya-Tate, N. et al. (1996) J Biol Chem 271, 14883-90.
  2. Bröer, S. (2008) Physiol Rev 88, 249-86.
  3. Bode, B.P. et al. (2002) Am J Physiol Gastrointest Liver Physiol 283, G1062-73.
  4. Fuchs, B.C. et al. (2007) Am J Physiol Cell Physiol 293, C55-63.
  5. Nicklin, P. et al. (2009) Cell 136, 521-34.
  6. Marin, M. et al. (2003) J Virol 77, 2936-45.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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