Cell Signaling Technology

Product Pathways - Translational Control

hnRNP Q/R Antibody #5351

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 75-82 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

hnRNP Q/R Antibody detects endogenous levels of hnRNP Q and hnRNP R proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human hnRNP Q. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and HT-29 cells using hnRNP Q/R Antibody.

Background

Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis, and mRNA transport from the nucleus to the cytoplasm (1-3). These two proteins are encoded by different genes but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AU-rich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulating RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (11).

  1. Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321.
  2. Burd, C.G. and Dreyfuss, G. (1994) Science 265, 615-21.
  3. Krecic, A.M. and Swanson, M.S. (1999) Curr Opin Cell Biol 11, 363-71.
  4. Passos, D.O. et al. (2006) Biochem Biophys Res Commun 346, 517-25.
  5. Moraes, K.C. et al. (2003) Biol Chem 384, 25-37.
  6. Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.
  7. Rossoll, W. et al. (2002) Hum Mol Genet 11, 93-105.
  8. Liu, H.M. et al. (2009) Virology 386, 249-56.
  9. Hassfeld, W. et al. (1998) Nucleic Acids Res 26, 439-45.
  10. Carty, S.M. and Greenleaf, A.L. (2002) Mol Cell Proteomics 1, 598-610.
  11. Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products