Cell Signaling Technology

Product Pathways - Translational Control

NME1/NDKA (D14H1) Rabbit mAb #5353

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk B Endogenous 16, 18 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NME1/NDKA (D14H1) Rabbit mAb detects endogenous levels of total NME1/NDKA protein. This antibody is predicted to cross-react with NME2/NDKB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Gly19 of human NME1/NDKA protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells lines using NME1/NDKA (D14H1) Rabbit mAb.

Background

The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKα1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKα1. This regulates AMPKα1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16).

  1. Lacombe, M.L. et al. (2000) J Bioenerg Biomembr 32, 247-58.
  2. Tee, Y.T. et al. (2006) Taiwan J Obstet Gynecol 45, 107-13.
  3. Ishikawa, N. et al. (2003) J Bioenerg Biomembr 35, 7-18.
  4. Paravicini, G. et al. (1996) Biochem Biophys Res Commun 227, 82-7.
  5. Reymond, A. et al. (1999) Oncogene 18, 7244-52.
  6. Subramanian, C. et al. (2001) Nat Med 7, 350-5.
  7. Zhu, J. et al. (1999) Proc Natl Acad Sci USA 96, 14911-8.
  8. Otsuki, Y. et al. (2001) Proc Natl Acad Sci USA 98, 4385-90.
  9. Palacios, F. et al. (2002) Nat Cell Biol 4, 929-36.
  10. Fan, Z. et al. (2003) Cell 112, 659-72.
  11. Postel, E.H. (2003) J Bioenerg Biomembr 35, 31-40.
  12. Wagner, P.D. and Vu, N.D. (2000) Biochem J 346 Pt 3, 623-30.
  13. Kimura, N. et al. (2000) J Bioenerg Biomembr 32, 309-15.
  14. Steeg, P.S. (2004) J Natl Cancer Inst 96, E4.
  15. Crawford, R.M. et al. (2006) Mol Cell Biol 26, 5921-31.
  16. Schaertl, S. et al. (1999) J Biol Chem 274, 20159-64.

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