Cell Signaling Technology

Product Pathways - Translational Control

Dicer (D38E7) Rabbit mAb #5362

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 220 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Dicer (D38E7) Rabbit mAb detects endogenous levels of total Dicer protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1133 of human Dicer.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and 293 cells using Dicer (D38E7) Rabbit mAb.

Background

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3).

  1. Hutvágner, G. and Zamore, P.D. (2002) Science 297, 2056-60.
  2. Lee, Y. et al. (2003) Nature 425, 415-9.
  3. Diederichs, S. and Haber, D.A. (2007) Cell 131, 1097-108.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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