Product Pathways - Adhesion
PSA/KLK3 (D6B1) XP® Rabbit mAb #5365
|5365S||100 µl (10 western blots)||---||In Stock||---|
|5365P||40 µl (4 western blots)||---||In Stock||---|
|5365||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
PSA/KLK3 (D6B1) XP® Rabbit mAb recognizes endogenous levels of total PSA/KLK3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe165 of human PSA/KLK3 protein.
Western blot analysis of extracts from LNCaP (PSA positive) and DU 145 (PSA negative) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow cytometric analysis of DU 145 (negative, blue) and LNCaP (positive, green) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb.
Confocal immunofluorescent analysis of LNCaP (positive, left) or DU 145 (negative, right) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 N-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (3,4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.