Product Pathways - Adhesion
PSA/KLK3 (D6B1) XP® Rabbit mAb #5365
PhosphoSitePlus® protein, site, and accession data: KLK3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC F | H | Endogenous | 29 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PSA/KLK3 (D6B1) XP® Rabbit mAb recognizes endogenous levels of total PSA/KLK3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe165 of human PSA/KLK3 protein.
Western Blotting
Western blot analysis of extracts from LNCaP (PSA positive) and DU 145 (PSA negative) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Flow Cytometry
Flow cytometric analysis of DU 145 (negative, blue) and LNCaP (positive, green) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of LNCaP (positive, left) or DU 145 (negative, right) cells using PSA/KLK3 (D6B1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Kallikrein 3 (KLK3), also known as Prostate Specific Antigen (PSA), is a member of the glandular kallikrein subfamily of serine proteases (1). It is produced by prostate epithelial cells and is secreted into prostatic ducts. Upon cleavage of 7 N-terminal amino acids (2), it is activated to liquefy semen in the seminal coagulum. Although PSA/KLK3 is produced in healthy individuals, investigators have found abnormally high levels in the blood of men with advanced prostate cancer (3,4).
- Watt, K.W. et al. (1986) Proc Natl Acad Sci U S A 83, 3166-70.
- Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91.
- Balk, S.P. et al. (2003) J Clin Oncol 21, 383-91.
- Thompson, I.M. et al. (2004) N Engl J Med 350, 2239-46.
Application References
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Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
