Product Pathways - Growth Factors/Cytokines
Human Oncostatin M (hOSM) #5367
Recombinant human Oncostatin M (hOSM) Ala26-Arg221 (Accession #NP_065391) was expressed in human 293 cells at Cell Signaling Technology.
Based on amino acid sequencing, greater than 85% of recombinant hOSM starts at Aa26 (AAIGS) and has a calculated MW of 19,249. The remainder starts at Ile28 (IGSCS). DTT-reduced and non-reduced protein migrate as 32 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hOSM. All lots are greater than 98% pure.
The bioactivity of recombinant hOSM was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 50-500 pg/ml.
The purity of recombinant hOSM was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hOSM and staining overnight with Coomassie Blue.
The proliferation of TF-1 cells treated with increasing concentrations of hOSM was assessed. After 48 hour treatment with hOSM, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
Western blot analysis of extracts from TF-1 cells, untreated or treated with hOSM for 10 minutes, using Phospho-Stat3 (Tyr705) (DA37) XP® Rabbit mAb #9145 (upper) and Stat3 Antibody #9132 (lower).
Less than 0.01 ng endotoxin/1 μg hOSM.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hOSM. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. In contrast, Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). OSM induces the activation the Jak2, Stat3, and Erk1/2 pathways (5).
- Malik, N. et al. (1989) Mol Cell Biol 9, 2847-53.
- Silver, J.S. and Hunter, C.A. (2010) J Leukoc Biol 88, 1145-56.
- Underhill-Day, N. and Heath, J.K. (2006) Cancer Res 66, 10891-901.
- Ichihara, M. et al. (1997) Blood 90, 165-73.
- Hintzen, C. et al. (2008) J Immunol 181, 7341-9.
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- 9145 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb
- 9872 Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)
For Research Use Only. Not For Use In Diagnostic Procedures.