Cell Signaling Technology

Product Pathways - Metabolism

OGT Antibody #5368

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 110 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

OGT Antibody recognizes endogenous levels of total OGT protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala686 of human OGT protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OGT Antibody.

Background

O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis, and transcription (2,3), and research studies have implicated this modification in cancer (1). The reversible protein modification by O-GlcNAc, which has been suggested to be a nutrient and stress sensor, is catalyzed by two highly conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) (4).

  1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
  2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
  3. Capotosti, F. et al. (2011) Cell 144, 376-88.
  4. Hart, G.W. et al. (2007) Nature 446, 1017-22.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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