Cell Signaling Technology

Product Pathways - Translational Control

Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) #5379

Applications Reactivity Sensitivity MW (kDa) Isotype
IP H M Endogenous 200 Rabbit IgG

Applications Key:  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) detects endogenous levels of total rictor protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence around Leu1121 of human rictor protein.

IP

IP

Immunoprecipitation of HeLa cell lysates using Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate) #3423 (lane 1) and Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) (lane 2). The western blot was probed using Rictor (D61H9) Rabbit mAb #9476.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Rictor (D16H9) Rabbit mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Rictor (D16H9) Rabbit mAb #9476.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).

  1. Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302.
  2. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101.
  3. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416.
  4. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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