Product Pathways - Neuroscience
Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb #5393
PhosphoSitePlus® protein, site, and accession data: DARPP-32
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R | Endogenous | 32 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5393:
- Western Blotting
Specificity / Sensitivity
Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb detects endogenous levels of DARPP-32 only when phosphorylated at Thr34. This antibody may also cross-react with an unidentified 40 kDa protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr34 of human DARPP-32.
Western Blotting
Western blot analysis of extracts from wild-type mouse striatum and DARPP-32 (-/-) mouse striatum, using Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb (upper) and CDK5 Antibody #2506 (lower). (Wild-type mouse striatum and DARPP-32 (-/-) mouse striatum was kindly provided by Dr. Paul Greengard and Helen Bateup, Rockefeller University, New York, NY).
Western Blotting
Western blot analysis of phospho-(Thr34)- and nonphospho-DARPP-32 recombinant proteins using Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb (upper) and DARPP-32 (19A3) Rabbit mAb #2306 (lower).
Background
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).
- Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.
- Bibb, J.A. et al. (1999) Nature 402, 669-671.
- Fienberg, A.A. et al. (1998) Science 281, 838-842.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
