Cell Signaling Technology

Product Pathways - Translational Control

KHSRP Antibody #5398

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 82 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

KHSRP Antibody detects endogenous levels of total KHSRP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human KHSRP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using KHSRP Antibody.

Background

KHSRP, also known as KSRP, is a KH domain-containing AU-rich element (ARE) binding protein (1). It recruits degradation machinery and activates mRNA turnover (2). This protein was previously shown to function as a regulator for splicing (3). KHSRP associates with both the Drosha and Dicer multiprotien complexes (4), and controls the biogenesis of some microRNAs by binding to the terminal loops of these microRNA precursors (3). KHSRP is found in neural and non-neural cell types, in the nucleus and the cytoplasm (4).

  1. Gherzi, R. et al. (2006) PLoS Biol 5, e5.
  2. Gherzi, R. et al. (2004) Mol Cell 14, 571-83.
  3. Min, H. et al. (1997) Genes Dev 11, 1023-36.
  4. Trabucchi, M. et al. (2009) Nature 459, 1010-4.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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