Cell Signaling Technology

Product Pathways - Protein Stability

DDB2 (D4C4) Rabbit mAb #5416

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M (R) (Mk) Endogenous 43 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DDB2 (D4C4) Rabbit mAb detects endogenous levels of total DDB2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ala174 of human DDB2.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji and HL-60 cells using DDB2 (D4C4) Rabbit mAb.

Background

Damaged DNA-Binding Protein (DDB) consists of a 127 kDa subunit (DDB-1) and a 48 kDa subunit (DDB-2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB) (1-3). In conjunction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway (4-6). Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients (7-10).

  1. Reardon, J.T. et al. (1993) J Biol Chem 268, 21301-8.
  2. Keeney, S. et al. (1993) J Biol Chem 268, 21293-300.
  3. Hwang, B.J. and Chu, G. (1993) Biochemistry 32, 1657-66.
  4. Chu, G. and Chang, E. (1990) Proc Natl Acad Sci USA 87, 3324-7.
  5. Hirschfeld, S. et al. (1990) Mol Cell Biol 10, 2041-8.
  6. Payne, A. and Chu, G. (1994) Mutat Res 310, 89-102.
  7. Chu, G. and Chang, E. (1988) Science 242, 564-7.
  8. Nichols, A.F. et al. (1996) J Biol Chem 271, 24317-20.
  9. Kataoka, H. and Fujiwara, Y. (1991) Biochem Biophys Res Commun 175, 1139-43.
  10. Keeney, S. et al. (1992) Mutat Res 273, 49-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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