Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Galectin-1/LGALS1 Antibody #5418

Applications Reactivity Sensitivity MW (kDa) Source
W H M R (Mk) Endogenous 15 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Galectin-1/LGALS1 Antibody detects endogenous levels of total galectin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu33 of human galectin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HL-60 and THP-1 cells using Galectin-1/LGALS1 Antibody.

Background

Galectins are a family of β-galactose binding proteins that are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and their carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions including cell adhesion (3), growth regulation (4), cytokine production (5), T-cell apoptosis (6), and immune responses (7). Galectin-1 (LGALS1) has been shown to be expressed in a wide range of tissues and cell types. The level and pattern of expression of galectin-1 has been shown to change during development (8). In addition to its apparent role in developmental processes, galectin-1 has been shown to be involved in central immune tolerance and may function in tumorigenesis by modulating the immune response to the tumor (9,10). Research studies have shown that galectin-1 expression is increased in several human cancers, suggesting a correlation with metastatic potential (10).

  1. Barondes, S.H. et al. (1994) Cell 76, 597-8.
  2. Barondes, S.H. et al. (1994) J Biol Chem 269, 20807-10.
  3. Offner, H. et al. (1990) J Neuroimmunol 28, 177-84.
  4. Wells, V. and Mallucci, L. (1991) Cell 64, 91-7.
  5. Filer, A. et al. (2009) Arthritis Rheum 60, 1604-14.
  6. Perillo, N.L. et al. (1995) Nature 378, 736-9.
  7. Cooper, D.N. et al. (1991) J Cell Biol 115, 1437-48.
  8. Puche, A.C. et al. (1996) Dev Biol 179, 274-87.
  9. van den Brûle, F. et al. (2003) Lab Invest 83, 377-86.
  10. Salatino, M. et al. (2008) Expert Opin Biol Ther 8, 45-57.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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