Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

ZFX (L28B6) Mouse mAb #5419

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 135 Mouse IgG2b

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

ZFX (L28B6) Mouse mAb detects endogenous levels of total ZFX protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human ZFX.

Western Blotting

Western Blotting

Western blot analysis of extracts from HL-60 and iPS cells using ZFX (L28B6) Mouse mAb.

Background

The ZFX (X-linked zinc finger protein) gene is expressed from the inactive X chromosome and is structurally similar to its homologue on the Y chromosome, ZFY (1). Transcripts of ZFX and ZFY encode proteins composed of a highly acidic amino-terminal domain and a carboxy-terminal zinc-finger motif that is commonly associated with nucleic acid-binding proteins (2). Both ZFY and ZFX are probable transcriptional activators and may function in sex determination (3). Multiple alternatively spliced transcript variants of ZFX, encoding different isoforms, have been identified and may be functionally distinct (2). Conditional gene targeting studies in mouse have suggested ZFX is also required for self-renewal of embryonic and hematopoietic stem cells (4). ZFX has also been suggested to play a role in proliferation and expansion of B cells, and could contribute to lymphocyte homeostasis (5).

  1. Schneider-Gädicke, A. et al. (1989) Cell 57, 1247-58.
  2. Schneider-Gädicke, A. et al. (1989) Nature 342, 708-11.
  3. Palmer, M.S. et al. (1990) Proc Natl Acad Sci U S A 87, 1681-5.
  4. Galan-Caridad, J.M. et al. (2007) Cell 129, 345-57.
  5. Arenzana, T.L. et al. (2009) Blood 113, 5857-67.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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