Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Bub1b (D32E8) Rabbit mAb #5421

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 130 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Bub1b (D32E8) Rabbit mAb detects endogenous levels of total Bub1b protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Lys370 of human Bub1b.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 and HeLa cells using Bub1b (D38E8) Rabbit mAb.

Background

The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase (2). Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6), and is required for functional microtubule-kinetochore interactions (7).

  1. Fukagawa, T. (2008) Front Biosci 13, 2705-13.
  2. Chen, R.H. (2007) J Biomed Sci 14, 475-9.
  3. Dai, W. et al. (2004) Cancer Res 64, 440-5.
  4. Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85.
  5. Baker, D.J. et al. (2004) Nat Genet 36, 744-9.
  6. Taylor, S.S. et al. (1998) J Cell Biol 142, 1-11.
  7. Logarinho, E. et al. (2008) Mol Biol Cell 19, 1798-813.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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