Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Emerin (D9B3) Rabbit mAb #5430

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 30 kDa Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Emerin (D9B3) Rabbit mAb detects endogenous level of total Emerin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Emerin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, 293, and MCF7 cell lines using Emerin (D9B3) Rabbit mAb.

Background

Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).

  1. Nagano, A. et al. (1996) Nat. Genet. 12, 254-259.
  2. Manilal, S. et al. (1998) Biochem. Biophys. Res. Commun. 249, 643-647.
  3. Clements, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 709-714.
  4. Lee, K.K. et al. (2001) J. Cell Sci. 114, 4567-4573.
  5. Bengtsson, L. and Wilson, K.L. (2006) Mol. Biol. Cell 17, 1154-1163.
  6. Holaska, J.M. et al. (2003) J. Biol. Chem. 278, 6969-6975.
  7. Haraguchi, T. et al. (2004) Eur. J. Biochem. 271, 1035-1045.
  8. Markiewicz, E. et al. (2006) EMBO J. 25, 3275-3285.
  9. Jacque, J.M. and Stevenson, M. (2006) Nature 441, 641-645.
  10. Holaska, J.M. and Wilson, K.L. (2006) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 288, 676-680.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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