Cell Signaling Technology

Product Pathways - DNA Damage

TERF2IP (D9H4) Rabbit mAb #5433

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 55 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

TERF2IP (D9H4) Rabbit mAb detects endogenous levels of total TERF2IP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human TERF2IP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TERF2IP (D9H4) Rabbit mAb.

Background

Telomeric repeat-binding factor 2-interacting protein (TERF2IP, also known as RAP1) is a component of the Shelterin Complex, a multi-protein complex that binds and organizes telomeres into T-loop structures to prevent them from being recognized by the cell as DNA double stranded breaks (1,2). The Shelterin Complex is composed of TERF2IP, TIN2 and TPP2 proteins, in addition to three DNA binding proteins that function to recruit the complex to telomeres: TRF1 and TRF2 bind double-stranded TTAGGG repeats found at telomeres, while the POT1 protein binds single-stranded TTAGGG repeats found at the very end of the telomeres (2). Together, these proteins function to protect telomeres and ensure proper replication and processing of chromosome ends. Recent studies have shown that TERF2IP is dispensable for maintenance of telomere length, organization of telomeric chromatin, and regulation of telomeric transcription (3,4). However, TERF2IP is required for inhibition of homology-directed repair (HDR), which can create undesirable telomeric sister chromatid exchange (3,4). In addition to its role in telomere maintenance, TERF2IP is also found in the cytoplasm, where it functions as an IκB kinase (IKK) adaptor protein and regulates NF-κB-dependent gene expression (5). TERF2IP forms a complex with IKKs and is critical for proper recruitment of IKKs to and activation of the p65 subunit of NF-κB. Elevated levels of TERF2IP have been found in breast cancer cells with NF-κB hyperactivity, and knockdown of TERF2IP sensitizes these cells to apoptosis, further identifying TERF2IP as a potential cancer therapeutic target (5).

  1. Li, B. et al. (2000) Cell 101, 471-83.
  2. de Lange, T. (2005) Genes Dev 19, 2100-10.
  3. Sfeir, A. et al. (2010) Science 327, 1657-61.
  4. Martinez, P. et al. (2010) Nat Cell Biol 12, 768-80.
  5. Teo, H. et al. (2010) Nat Cell Biol 12, 758-67.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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