Cell Signaling Technology

Product Pathways - Development

RBPSUH Antibody #5442

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 61 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RBPSUH Antibody detects endogenous level of total RBPSUH protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu111 of human RBPSUH protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cells using RBPSUH Antibody.

Background

RBPSUH (Recombining Binding Protein, SUppressor of Hairless), also termed RBP-J or CSL, is the DNA-binding component of the transcription complex regulated by canonical Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation of Notch receptors, the Notch intracellular domain (NICD) translocates to the nucleus and binds to RBPSUH. This displaces the co-repressor complex and replaces it with a transcription activation complex that includes Mastermind-like (MAML) proteins and histone acetylase p300, leading to transcriptional activation of Notch target genes (1-3). RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5).

  1. Ehebauer, M. et al. (2006) Sci STKE 2006, cm7.
  2. Borggrefe, T. and Oswald, F. (2009) Cell Mol Life Sci 66, 1631-46.
  3. Kopan, R. and Ilagan, M.X. (2009) Cell 137, 216-33.
  4. Henkel, T. et al. (1994) Science 265, 92-5.
  5. Strobl, L.J. et al. (1997) Immunobiology 198, 299-306.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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