Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Ikaros Antibody #5443

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P F H M R Endogenous 50-70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ikaros Antibody detects endogenous levels of total Ikaros protein. It is expected to recognize all isforms of Ikaros protein produced by differential splicing.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro329 of human Ikaros protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western analysis of extracts from various cell lines using Ikaros Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using Ikaros Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ikaros Antibody. Note staining of leukocytes.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using Ikaros Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using Ikaros Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red).

Background

Ikaros family proteins, characterized by the presence of an N-terminal zinc finger DNA-binding domain and a C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. The Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers and heterodimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2).Ikaros, also known as Ikaros family zinc finger protein 1 (IKZF1) and Lymphoid transcription factor 1 (LYF-1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Research studies have shown that imbalanced expression of different Ikaros isoforms, as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7).

  1. Perdomo, J. et al. (2000) J Biol Chem 275, 38347-54.
  2. Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
  3. Georgopoulos, K. et al. (1997) Annu Rev Immunol 15, 155-76.
  4. Nichogiannopoulou, A. et al. (1998) Semin Immunol 10, 119-25.
  5. Tonnelle, C. et al. (2002) Leuk Lymphoma 43, 29-35.
  6. Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
  7. Martinelli, G. et al. (2009) J Clin Oncol 27, 5202-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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